Assist in conducting research in biochemistry, cell and molecular biology.
Manage mouse colonies and perform experiments with live animals.
Help in the everyday operation of an active NIH-funded lab and contributing to the research program.
Interface with students, other researchers and other laboratories.
Primary Duties & Responsibilities of this position are as follows:
Conduct experiments: Perform various experiments involving live mice as well as biochemical analyses of mouse tissues, such as Western immunoblotting, glycogen and protein determination. Perform basic cell culture experiments and maintain cell lines. Molecular cloning. Data analysis, statistics and preparation of data for publication or presentation. Keep precise records of experiments in a laboratory notebook.
Laboratory management: Train laboratory personnel in various techniques and analyses including biochemical and molecular biology techniques, protein determination, cultured cell analyses. Serve as a resource to other laboratory personnel. Order supplies and equipment for the laboratory and obtain product quotes as needed. Survey for radioactive contamination, maintain inventory, take care of Geiger Counter calibration, as required by the Radiation Safety Office. Maintain records for safety inspections and monitor best practices for the laboratory as required.
Management of mouse colonies: Set up breeding cages, as required, in consultation with faculty. Genotype by PCR from mouse biopsies. Wean, euthenize, treat and generally take care of several different mouse lines. Maintain detailed records of mouse census in Excel. Interface with LARC and LARC personnel.
Continuing education: Maintain knowledge of relevant scientific trends through readings and seminars.
Master's degree in science; OR bachelor's degree in biochemistry, chemistry, biology, or a related field and three years of experience in a biochemical and biology laboratory.
Experience working with live rodents, primarily mice. Experience in basic biochemical assays, including Western blots, enzymatic assays, PCR, and protein determination. Experience in cell culture and molecular biology. Basic computer software skills (MS Office Word, Excel, Powerpoint), including some informatics and statistical analyses. Excellent communication skills. Highly self-motivated, well-organized, and willing to learn. Ability to interact well with others and work as part of a team.
Working Conditions / Demands
Must be able to perform the essential duties of the position with or without an accomodation.
Equipment utilized: Equipment utilized: scintillation counter, microscopes, PCR machines, electrophoresis apparatus, western blot apparatus, centrifuges, spectrophometer/plate reader, biosafety cabinet, and standard laboratory small equipment.
Salary Plan: PAE
Salary Grade: 3RS
Job Function: Research
Due to the COVID-19 pandemic, there may be differences in the working conditions as advertised in our standard job postings (e.g., the ability to travel from one campus to another, etc.). If you are invited for an interview, please discuss your questions or concerns regarding the working conditions at that time.
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