PED-GENETICS RESEARCH (IN-PGNB-IUINA)

The Herman B Wells Center for Pediatric Research conducts basic science and translational research within the Department of Pediatrics at Indiana University School of Medicine. The Wells Center houses more than 200 faculty investigators, staff and trainees seeking answers to the most pressing questions related to childhood illness.

With the collaboration between scientists and physicians, the center aims to increase knowledge of causes and mechanisms of serious pediatric diseases. Our goal is to develop innovative approaches to the diagnosis and treatment of sick children in Indiana and beyond.

The Wells Center encompasses a breadth of multidisciplinary expertise in biochemistry and molecular biology, immunology, molecular genetics, and cellular and integrative physiology--making it a uniquely collaborative environment.

This position will assist the Principal Investigator in conducting research in Cardiovascular Research Group within the Wells Center for Pediatric Research. This research is focused on examining the role of non-coding RNAs in human heart development and disease using human embryonic stem (ES) cell and induced pluripotent stem (iPS) cells. This research makes extensive use of molecular biology, cell biology techniques and genetic models.

Primary Duties and Responsibilities:

- Perform molecular biology experiments including, but not limited to, plasmid construction, bacterial culture, stem cell culture, transfection, virus packaging, RNA isolation, flow cytometry analysis, qRT-PCR, western blotting and small animal handling.

- Maintain mouse colonies. Consists of setting up and maintaining breeder cages, maintaining nonbreeding cages, animal handling, such as weaning, ear notching, tail snips, dosing, and maintaining colony records.

- General laboratory management duties, including order lab supplies, maintain inventory and reagents, assist with the maintenance of regulatory monitoring and paperwork (IACUC, IRB, Biosafety, Laboratory Safety, and Radiation Safety), set-up and maintenance of laboratory equipment, and schedule laboratory meetings and presentations, as needed.

Bachelor's degree in science.

Laboratory experience and the ability to learn quickly. Ability to function independently and conduct experiments. Experience in mouse and small animal colony maintenance and/or small animal surgery. Ability to problem solve.

Two years of lab experience.

Indianapolis, Indiana

Salary Plan: RS

Salary Grade: 07

FLSA: Nonexempt

Job Function: Research

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